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human melanocytes nhm  (PromoCell)


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    Structured Review

    PromoCell human melanocytes nhm
    Figure 1. Melanoma transformation and progression are associated with stiffness decrease. (a) (Top left panel), overall stiffness measurement of whole human skin reconstructs (HSR) con- taining primary <t>melanocytes</t> <t>(NHM)</t> or melanoma cells. (Top right panel), stiffness measurement of melanoma cells in HSR cryosections. (Bottom panels), imaging of the corresponding HSR sections. (b) Global measurement of skin stiffness of medaka fish mitf::Xmrk/+ (left panel) or mitf::Xmrk/+ p53-/- (right panel) on healthy areas compared to areas with melanoma. Stiffness mea- surements were conducted on the flank or in dorsal areas of medakas. (c) (Upper left panel), optical and mechanical correlative images showing the rigidity of the skin at the interface between healthy area (white area (optical)) and melanoma area (black area (optical)) of the whole mitf::Xmrk/+ medaka. (Bottom left panel), tomographic reconstruction of the area scanned by AFM (projection in xz).
    Human Melanocytes Nhm, supplied by PromoCell, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human melanocytes nhm/product/PromoCell
    Average 93 stars, based on 5 article reviews
    human melanocytes nhm - by Bioz Stars, 2026-02
    93/100 stars

    Images

    1) Product Images from "Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion."

    Article Title: Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion.

    Journal: Cancers

    doi: 10.3390/cancers16040694

    Figure 1. Melanoma transformation and progression are associated with stiffness decrease. (a) (Top left panel), overall stiffness measurement of whole human skin reconstructs (HSR) con- taining primary melanocytes (NHM) or melanoma cells. (Top right panel), stiffness measurement of melanoma cells in HSR cryosections. (Bottom panels), imaging of the corresponding HSR sections. (b) Global measurement of skin stiffness of medaka fish mitf::Xmrk/+ (left panel) or mitf::Xmrk/+ p53-/- (right panel) on healthy areas compared to areas with melanoma. Stiffness mea- surements were conducted on the flank or in dorsal areas of medakas. (c) (Upper left panel), optical and mechanical correlative images showing the rigidity of the skin at the interface between healthy area (white area (optical)) and melanoma area (black area (optical)) of the whole mitf::Xmrk/+ medaka. (Bottom left panel), tomographic reconstruction of the area scanned by AFM (projection in xz).
    Figure Legend Snippet: Figure 1. Melanoma transformation and progression are associated with stiffness decrease. (a) (Top left panel), overall stiffness measurement of whole human skin reconstructs (HSR) con- taining primary melanocytes (NHM) or melanoma cells. (Top right panel), stiffness measurement of melanoma cells in HSR cryosections. (Bottom panels), imaging of the corresponding HSR sections. (b) Global measurement of skin stiffness of medaka fish mitf::Xmrk/+ (left panel) or mitf::Xmrk/+ p53-/- (right panel) on healthy areas compared to areas with melanoma. Stiffness mea- surements were conducted on the flank or in dorsal areas of medakas. (c) (Upper left panel), optical and mechanical correlative images showing the rigidity of the skin at the interface between healthy area (white area (optical)) and melanoma area (black area (optical)) of the whole mitf::Xmrk/+ medaka. (Bottom left panel), tomographic reconstruction of the area scanned by AFM (projection in xz).

    Techniques Used: Transformation Assay, Imaging



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    Figure 1. Melanoma transformation and progression are associated with stiffness decrease. (a) (Top left panel), overall stiffness measurement of whole human skin reconstructs (HSR) con- taining primary <t>melanocytes</t> <t>(NHM)</t> or melanoma cells. (Top right panel), stiffness measurement of melanoma cells in HSR cryosections. (Bottom panels), imaging of the corresponding HSR sections. (b) Global measurement of skin stiffness of medaka fish mitf::Xmrk/+ (left panel) or mitf::Xmrk/+ p53-/- (right panel) on healthy areas compared to areas with melanoma. Stiffness mea- surements were conducted on the flank or in dorsal areas of medakas. (c) (Upper left panel), optical and mechanical correlative images showing the rigidity of the skin at the interface between healthy area (white area (optical)) and melanoma area (black area (optical)) of the whole mitf::Xmrk/+ medaka. (Bottom left panel), tomographic reconstruction of the area scanned by AFM (projection in xz).
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    Image Search Results


    Figure 1. Melanoma transformation and progression are associated with stiffness decrease. (a) (Top left panel), overall stiffness measurement of whole human skin reconstructs (HSR) con- taining primary melanocytes (NHM) or melanoma cells. (Top right panel), stiffness measurement of melanoma cells in HSR cryosections. (Bottom panels), imaging of the corresponding HSR sections. (b) Global measurement of skin stiffness of medaka fish mitf::Xmrk/+ (left panel) or mitf::Xmrk/+ p53-/- (right panel) on healthy areas compared to areas with melanoma. Stiffness mea- surements were conducted on the flank or in dorsal areas of medakas. (c) (Upper left panel), optical and mechanical correlative images showing the rigidity of the skin at the interface between healthy area (white area (optical)) and melanoma area (black area (optical)) of the whole mitf::Xmrk/+ medaka. (Bottom left panel), tomographic reconstruction of the area scanned by AFM (projection in xz).

    Journal: Cancers

    Article Title: Cancer Cell Biomechanical Properties Accompany Tspan8-Dependent Cutaneous Melanoma Invasion.

    doi: 10.3390/cancers16040694

    Figure Lengend Snippet: Figure 1. Melanoma transformation and progression are associated with stiffness decrease. (a) (Top left panel), overall stiffness measurement of whole human skin reconstructs (HSR) con- taining primary melanocytes (NHM) or melanoma cells. (Top right panel), stiffness measurement of melanoma cells in HSR cryosections. (Bottom panels), imaging of the corresponding HSR sections. (b) Global measurement of skin stiffness of medaka fish mitf::Xmrk/+ (left panel) or mitf::Xmrk/+ p53-/- (right panel) on healthy areas compared to areas with melanoma. Stiffness mea- surements were conducted on the flank or in dorsal areas of medakas. (c) (Upper left panel), optical and mechanical correlative images showing the rigidity of the skin at the interface between healthy area (white area (optical)) and melanoma area (black area (optical)) of the whole mitf::Xmrk/+ medaka. (Bottom left panel), tomographic reconstruction of the area scanned by AFM (projection in xz).

    Article Snippet: A melanocyte growth medium kit was used for normal human melanocytes (NHM) (Promocell, Heidelberg, Germany).

    Techniques: Transformation Assay, Imaging

    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis

    doi: 10.1016/j.ccell.2019.12.007

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: NHM_N5 (Melanocytes) NHEM Juvenile, cell pellet in RNAlater , Promocell , Cat. # C-14040; lot 4070201.1.

    Techniques: Produced, Subcloning, Recombinant, Western Blot, Autoradiography, RNA Extraction, SYBR Green Assay, Sample Prep, Chromatin Immunoprecipitation, TA Cloning, Sequencing, Cell Culture, DNA Methylation Assay, shRNA, Negative Control, Positive Control, Mutagenesis, Software

    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis

    doi: 10.1016/j.ccell.2019.12.007

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: NHM_N4 (Melanocytes) NHEM PD#10 (Population Doubling) , Promocell , Cat. # C-12400; lot 429Z015.3.

    Techniques: Produced, Subcloning, Recombinant, Western Blot, Autoradiography, RNA Extraction, SYBR Green Assay, Sample Prep, Chromatin Immunoprecipitation, TA Cloning, Sequencing, Cell Culture, DNA Methylation Assay, shRNA, Negative Control, Positive Control, Mutagenesis, Software

    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: Epigenetic silencing of CDR1as drives IGF2BP3-mediated melanoma invasion and metastasis

    doi: 10.1016/j.ccell.2019.12.007

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: NHM_N6 (Melanocytes) NHEM-M2 Juvenile, cell pellet in RNAlater , Promocell , Cat. # C-14042; lot 5071302.2.

    Techniques: Produced, Subcloning, Recombinant, Western Blot, Autoradiography, RNA Extraction, SYBR Green Assay, Sample Prep, Chromatin Immunoprecipitation, TA Cloning, Sequencing, Cell Culture, DNA Methylation Assay, shRNA, Negative Control, Positive Control, Mutagenesis, Software